Colección INTI +


Título: Functional evaluation of ToxA promoter in Trichoderma reesei Rut- C30
Fuente: BioTechnology : an Indian Journal, 12(1)
Autor/es: Ortiz, Gastón E.; Blasco, Martín; Albertó, Edgardo
Materias: Proteínas; Fluorimetría; Hongos; Evaluación; Biotecnología; Análisis microbiológico
Editor/Edición: Trade Science Inc.; 2016
Licencia: info:eu-repo/semantics/openAccess;
Afiliaciones: Ortiz, Gastón E. Instituto Nacional de Tecnología Industrial (INTI-Biotecnología Industrial); Argentina
Blasco, Martín. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico Chascomús (IIB-INTECH-UNSAM-CONICET); Argentina
Albertó, Edgardo. Instituto Nacional de Tecnología Industrial (INTI-Biotecnología Industrial); Argentina

Resumen: In order to expand the collection of promoters studied in Trichoderma reesei Rut-C30, a functionalstudy of the ToxA promoter was carried out by analysis of GFP expression in T. reesei Rut-C30. For this purpose, the binary pCBCT expression vector was employed to transform T.reesei Rut- C30. The transformants obtained were evaluated by means of fluorescence microscopy, fluorometry, dot blot and Western blot analysis. The low levels of cytoplasmic GFP protein in fungal hyphae suggest that ToxA promoter works as a weak constitutive promoter which can drive successfully the expression of heterologous proteins in T. reesei Rut-C30
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